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human lymphocyte separation medium  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science human lymphocyte separation medium
    Human Lymphocyte Separation Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 632 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lymphocyte+separation+medium/Lymphocyte+Separation+Medium/pm41964031-120-12-16
    Average 96 stars, based on 632 article reviews
    human lymphocyte separation medium - by Bioz Stars, 2026-09
    96/100 stars

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    Isolation:

    Article Title: Chronic HBV infection impairs the glucose metabolism and effector function of NK cells via HBsAg/IL-15/mTOR axis.
    Article Snippet: .. Isolation of human PBMCs and NK cells Human PBMCs were separated from peripheral blood by density gradient centrifugation at room temperature (RT) using Human Lymphocyte Separation Medium (Solarbio, Beijing, China). .. NK cells from PBMCs were sorted according to the instructions of MojoSortTM Human NK Cell Isolation Kit (Biolegend, USA).

    Article Title: DKC1: a robust prognostic factor and potential therapeutic target in multiple myeloma.
    Article Snippet: Cells were maintained in a humidified incubator at 37°C with 5% CO2 and monitored for mycoplasma infection every six months by using MycoBlue Mycoplasma Detector (D101-02, Vazyme, Nanjing, China). .. Peripheral blood samples from three healthy donors were collected after informed consent, and peripheral blood mononuclear cells (PBMCs) were isolated using human lymphocyte separation medium (Ficoll, P8900, Solarbio, Beijing, China). ..

    Article Title: IGFBP2 promotes immunosuppression by regulating macrophage PD-L1 expression in NEUROD1-high small cell lung cancer.
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density-gradient centrifugation using Human Lymphocyte Separation Medium (Solarbio, China; CatR1010): 750×g for 25 min at room temperature. ..

    Article Title: Differential expression profiles of lncRNAs and a preliminary study on the mechanism of lncRNA FAM225A in triple seronegative myasthenia gravis
    Article Snippet: .. We isolated PBMCs from the peripheral blood using human lymphocyte separation medium (Solarbio, China) following the manufacturer’s instructions and extracted total RNA using TRIzol ® Reagent (Invitrogen, USA). .. NanoDrop ® ND-1000 was used to assess RNA quality and quantity.

    Article Title: FAM135B Deficiency Inhibits Cytotoxic T-Cell Activity in Triple-negative Breast Cancer by Blocking the IFI16-dependent STING Pathway
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMCs) were isolated from serum samples obtained from healthy volunteers using human lymphocyte separation medium (P8610, Solarbio). .. PBMCs were maintained in RPMI 1640 medium supplemented with 10% FBS and 10 ng/mL human IL-2 (Med Chem Express) for 3 days before coculture with tumor cells.

    Article Title: FAM135B Deficiency Inhibits Cytotoxic T-cell Activity in Triple-Negative Breast Cancer by Blocking the IFI16-Dependent STING Pathway
    Article Snippet: .. Human peripheral blood mononuclear cells (PBMC) were isolated from serum samples obtained from healthy volunteers using human lymphocyte separation medium (P8610, Solarbio). .. PBMCs were maintained in RPMI-1640 medium supplemented with 10% FBS and 10 ng/mL human IL2 (MedChemExpress) for 3 days before coculture with tumor cells.

    Article Title: DKC1: a robust prognostic factor and potential therapeutic target in multiple myeloma
    Article Snippet: Cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and monitored for mycoplasma infection every six months by using MycoBlue Mycoplasma Detector (D101-02, Vazyme, Nanjing, China). .. Peripheral blood samples from three healthy donors were collected after informed consent, and peripheral blood mononuclear cells (PBMCs) were isolated using human lymphocyte separation medium (Ficoll, P8900, Solarbio, Beijing, China). ..

    Gradient Centrifugation:

    Article Title: Chronic HBV infection impairs the glucose metabolism and effector function of NK cells via HBsAg/IL-15/mTOR axis.
    Article Snippet: .. Isolation of human PBMCs and NK cells Human PBMCs were separated from peripheral blood by density gradient centrifugation at room temperature (RT) using Human Lymphocyte Separation Medium (Solarbio, Beijing, China). .. NK cells from PBMCs were sorted according to the instructions of MojoSortTM Human NK Cell Isolation Kit (Biolegend, USA).

    Article Title: Chronic HBV infection impairs the glucose metabolism and effector function of NK cells via HBsAg/IL-15/mTOR axis
    Article Snippet: .. Human PBMCs were separated from peripheral blood by density gradient centrifugation at room temperature (RT) using Human Lymphocyte Separation Medium (Solarbio, Beijing, China). .. NK cells from PBMCs were sorted according to the instructions of MojoSortTM Human NK Cell Isolation Kit (Biolegend, USA).

    Centrifugation:

    Article Title: IGFBP2 promotes immunosuppression by regulating macrophage PD-L1 expression in NEUROD1-high small cell lung cancer.
    Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density-gradient centrifugation using Human Lymphocyte Separation Medium (Solarbio, China; CatR1010): 750×g for 25 min at room temperature. ..



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